NS genes of muscovy duck reovirus S14 and C4 were amplified by RT_PCR with the primers designed by the reported avian and muscovy duck reoviruses. The PCR products were cloned to the pMD18_T vector and identified by enzyme cutting analysis.

• 参考GenBank禽呼肠孤病毒 (AvianReovirus,ARV)和番鸭呼肠孤病毒 (MuscovyDuckReovirus,MDRV)非结构基因 (NS)序列设计合成一对引物 ,对番鸭呼肠孤病毒S14和C4株NS基因进行RT_PCR扩增 ,克隆到pMD18_T载体中 ,并对克隆产物进行酶切鉴定和测序 ;