Mouse obese gene was amplified by PCR and subcloned into prokaryotic expression vector pET-28a(+) to construct the recombinant pET-28a-lep. Transforming pET-28a-lep into E.

• 本研究以克隆有小鼠肥胖基因的质粒pMET mouse leptin为模板，PCR扩增小鼠的ob基因，定向克隆至原核表达载体pET-28a(+)，构建重组表达质粒pET-28a-lep。