Most enzymes of gluconeogenesis are cytosolic, but pyruvate carboxylase and glucose 6-phosphatase are located in the mitochondrial matrix and bound to the smooth endoplasmic reticulum, respectively.

• 大部分糖异生作用的酶是胞质溶胶酶 , 但丙酮酸羧化酶和葡萄糖 6- 磷酸酶则分别位于线粒体基质和结合在光滑内质网上。