Methods.Cells of the nucleus pulposus and inner and outer anulus fibrosus were enzymatically isolated from boine coccygeal tissue and precultured in alginate beads for 14 days.
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方法:髓核细胞和内外纤维环通过酶与牛尾骨组织隔绝并在14天前培养于藻酸盐中。
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