Methods.Cells of the nucleus pulposus and inner and outer anulus fibrosus were enzymatically isolated from boine coccygeal tissue and precultured in alginate beads for 14 days.

• 方法：髓核细胞和内外纤维环通过酶与牛尾骨组织隔绝并在14天前培养于藻酸盐中。