MethodsSome reaction condition was optimized by amplifying technology and method of PCR based on isolating genomic DNA from D.

• 方法在提取纯化美花石斛基因组DNA的基础上,利用PCR扩增技术和方法,对Mg2+,dNTP,模板DNA,引物,Taq聚合酶等反应条件进行优化。