Methods:The recombinant plasmid pIRApoptinHNIL18 was introduced into human colon carcinoma cells HCT-116 by liposome-mediated transfection. The expression of the exogenous genes was detected by Western blotting and RT-PCR.

• 方法:重组质粒pIRApoptinHNIL18通过脂质体介导法体外转染人结肠癌细胞HCT-116,通过Western blotting和RT-PCR检测外源基因表达;