Methods:The mice spleen cells were fused with SP2/0 cells to prepare the hybridoma cell in different culture medium.The acting time was changed when chromosome was prepared.

• 方法:取小鼠脾细胞与骨髓瘤细胞SP2/0常规融合,用不同的培养基制备杂交瘤细胞,并在制备杂交瘤染色体时改变秋水仙素作用的时间。