Methods:The guinea pigs were killed by decapitation following exposure to the moderate BUP. [Ca~(2+)]i in the OHCs was examined with Fluo-3,a fluorescent probe for [Ca~(2+)]i under laser scanning confocal microscope(LSCM).

• 方法:在激光共聚焦显微镜下,用钙敏荧光探针Fluo-3作为指示剂,观察豚鼠暴露中等强度实验性BUP后耳蜗外毛细胞[Ca2+]i的变化。