Methods:The fermentation extracts were isolated and purified from endophyte HCCB00189 by solvent extraction,column chromatography and preparative HPLC,respectively.

• 方法:采用溶剂萃取、硅胶柱层析及制备HPLC等方法,对活性菌株的发酵物进行分离,并根据理化性质和光谱分析对化学结构进行鉴定。