Methods:The 293T cells was cotransfected with PDG and an AAV plasmid containing human apoAIcDNA to produce infectious rAAV, and it was purified by non ionic iodixanol gradients followed by heparin affinity chromatography.

• 方法 :以质粒 PDG和含有人 apo AIc DNA的AAV载体共转染 2 93T细胞 ,继以 Iodixanol密度梯度离心结合肝素亲和层析法分离、纯化 ,斑点杂交鉴定 r AAV病毒颗粒数 ;