Methods:Radix Clematidis was smashed first,extracted with 70% ethanol and purified with D101 macroporous absorptive resin,and eluted with 10% ammonia water,distilled water,20% ethanol and 70% ethanol.

• 方法:将威灵仙粉粹后用70%25乙醇渗滤提取,收集浓缩提取液后,上D101型大孔树脂,依次用10%25氨水、水、20%25乙醇洗脱杂质,最后以70%25乙醇洗脱有效部位,得总皂苷提取物,用齐墩果酸作对照,检测其含量。