Methods:Polymerase chain reaction(PCR) based microsatellite polymorphism analyses were performed to detect LOH on chromosome 8 and 22, fluorescence-labeled primers and Perkin Elmer 377 DNA Sequencer were applied.

• 方法 :应用聚合酶链反应 (PCR)方法 ,采用荧光标记的引物和 377型DNA序列自动分析仪 ,分别分析了 2 1例GBM 8号、2 2号染色体上 14个、7个微卫星多态性标记的LOH。