Methods:PCR based microsatellite polymorphism analyses were performed to detect LOH on chromosome 6, fluorescence labeled primers and Perkin Elmer 377 DNA Sequencer were applied.

• 方法 :应用聚合酶链反应 (PCR)方法 ,采用荧光标记的引物及 377型DNA序列自动分析仪 ,分析了 2 1例GBM患者 6号染色体上 2 0个微卫星多态性标记的LOH。