Methods:Oligonucleotide fragment of Brugia malayi synthesized by genetic engineering technique was labeled with 32P and used as probe.The B.malayi larvae in mosquito was detected with dot blot.

• 方法：利用基因工程技术合成马来丝虫寡核苷酸片段，经32P标记后作为探针，以斑点杂交法检测蚊体内马来丝虫幼虫。