Methods:Cell culture technique, MTT colorimetric assay, ALP activity assay, Commasie brilliant blue stain and Dahl McGec Russell s alizarin red stain for calcium were used.

• 方法:采用细胞培养、MTT比色测定法、碱性磷酸酶(ALP)测定法、考马斯亮蓝法和茜素红染色法。