Methods:ABC methods of immunohistochemistry and image analysis technique were used to study the changes of 5-HT in nucleus raphe dorsalis after cerebral ischemia and reperfusion in different time.

• 方法：用免疫组织化学ABC法及图象分析对沙土鼠脑缺血不同时间以及缺血10分钟后再灌不同时间其中缝背核神经元5-HT的变化进行研究。