Methods: take the bursa tissue of the 10-day-old normalBroiler ROSS308 and use Paraffin TUNEL Assayoptimized in the concentration of proteinase K , DAB chromogenic time and Hematoxylin staining time .

• 方法：用10日龄正常ROSS308肉仔鸡法氏囊组织，采用石蜡切片TUNEL法并对蛋白酶K的浓度、DAB的显色时间、苏木素的复染时间进行了优化。