Methods: The specific BDNF sequence was cloned into the plasmid of pAAV-MCS in AAV helper-Free system to construct the BDNF expression plasmid pAAV-MCS-BDNF.

• 方法应用基因克隆技术将大鼠BDNF的cDNA基因序列克隆入腺病毒质粒pAAV-MCS,PCR酶切测序鉴定序列。