Methods: The 681 bp target gene was amplified from heart tissue of young rats with RT PCR, fusion gene of huIL 2/myosin was constructed by splicing with huIL 2 cDNA using ligation methods and its RV was constructed.

• 方法 :以 RT- PCR方法从幼龄大鼠心肌组织中扩增出 6 81bp的目的基因 ,与 hu IL - 2 c DNA序列拼接 ,构建融合基因 hu IL - 2 / m yosin及其逆转录病毒载体。