Methods: Retrovirus was packaged in vitro with PA317 cells and Jurkat cell was transducted with collected virus; The expressions of bcl 2 mRNA and protein were assayed by RT PCR and Western blotting, respectively.
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方法:PA317细胞包装逆转录病毒,病毒转导Jurkat细胞后用RT?PCR及Western印迹法检测bcl?2mRNA及蛋白表达水平;
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