Methods: Recombinant plasmid pFHTNS3 was constructed by inserting HGV NS3 gene into transposing vector pFastBac HTa. pFHTNS3 plasmid was further transformed into DH10Bac competent cells.

• 方法 :将 HGV NS3基因片段定向克隆至转座载体 p Fast Bac HTa,转化 DH10 Bac感受态细胞 ,37℃振荡培养 4h使发生转座 ,用抗生素平皿筛选重组 bacmid。