Methods: PBMC was incubated with rhCSa, PMA, Chelerythrine, CalphostinC, Dequalinium for 24 hours, the IL-8 in the supemate was detected by ELBA.

• 方法:将PBMC分别与rhC5a、PMA、Cheleythrine、 Calphostin C、Dequalinium孵育24 h,取上清用法测定IL-8。