Methods: Murine lung endothelial cells were subcultured and incubated with HMME for 24 hours. Then the cells were stained with Rhodamine 123 for mitochondrion? Lucifer yellow for lysosome?

• 方法 :传代培养鼠肺毛细血管内皮细胞 ,将血卟啉单甲醚 (HMME)与内皮细胞共同孵育 2 4小时后 ,加入线粒体探针Rhodamine 12 3、内质网探针DioC6 ( 3 )和溶酶体探针Luciferyellow分别对细胞器染色。