Methods: Lymph nodes taken from lung cancer patient is dissociated into single cell suspension by mechanical method and cultured in culture medium added CD3 mAb and a little dose IL-2.

• 方法：取肺癌清扫淋巴结，用机械方法分离成单细胞悬液，加CD3单克隆抗体和小剂量IL-2共同培养，每隔1天用流式细胞术进行细胞表型分析及用台盼蓝活细胞计数法观察细胞增殖情况。