Methods: Isolated toad gastrocnemius stimulated by electricity as the fatigue model, the duration and amplitude of muscle contraction were recorded.

• 方法:采用脉冲式电流直接刺激离体蟾蜍腓肠肌作为疲劳模型,观察肌肉收缩力、达最大收缩力所需时间以及肌肉收缩持续时间的变化。