Methods: Intraperitoneal injection of formalin-fixed APP-1 strain vaccine antigen. ELISA antibody titer of serum LPS and choose the mouse with the highest value for cell fusion.

• 方法:腹腔注射APP-1标准株的甲醛固定抗原免疫BALB/c小鼠,ELISA检测血清中LPS抗体滴度并选择值最高的小鼠用于细胞融合,且于融合前3天加强免疫1次。