Methods: Human, Yorks hire pig or SD rat fibroblasts were cultured in DMEM with fetal boven serum(P BS) at the concentrations(ml/L) of 100 (A), 50 (B), 20 (C) or 20 supplemente d with 0.025 g/L boven pituitary extract (BPE)(D).

• 方法 :分别配制体积分数为 10 0、50、2 0ml/L血清和 2 0ml/L血清 +牛垂体提取物 (BPE ；0 .；0 2 5g/L) 4种培养基来培养人、York猪、SD鼠的真皮成纤维细胞；通过MTT比色和BrdU检测观察细胞的生长状态。