Methods: Freezing the left parietal bone to -196 to cause a frozen injury, injected % Evans Blue (2ml/kg) into the tail vein 10 minutes later.

• 方法：大鼠颅骨外用-196℃致冷冻性颅脑损伤。 伤后10分钟从尾静脉注入2%25伊文思蓝（2ml/kg）。