Methods: Establishing ache models, observe on the content of 5-HT, 5-HTAA, NE in hypothalamus of rats by chemical methods to study effect of extraction of natrix tigrina lateralis by alcohol to which.

• 方法：建立伤害性疼痛动物模型，通过化学荧光法，测定大鼠下丘脑组织中5-HT、5-HTAA、NE含量，观察虎斑游蛇不同浓度醇提物对其含量的影响。