Methods: D116H-ANG - pAxCAwt cosmid DNA and DNA-TPC were mixed and co-transfected to the 293 cell by calcium phosphate coprecipitation.

• 方法：ANG衍生物D116H-ANG重组粘粒与腺病毒DNA末端肽复合物混合后以磷酸钙共沉淀法转染293人胚肾细胞株细胞。