Methods: Cytopathic effect(CPE)was investigated,50% tissue culture infection dose(TCID50)was titrated on vero cells monolayer and cardiac histological lesions were investigated.

• 方法 :观察细胞病变效应 (CPE) ,常规微量法滴定病毒的50%25组织细胞感染量 (TCID50)以及观察感染小鼠心肌组织的病理改变。