Methods: Column chromatography and recrystallization were adopted to isolate and purify neferine, while its structure and purity were determined by UV, IR, NMR, MS, TLC and HPLC analysis.

• 方法柱层析分离,重结晶纯化甲基莲心碱,UV,IR,NMR,MS,HPLC及TLC等方法确定甲基莲心碱的结构和纯度。