Methods: Collected human hydrosalpin fluid,female mice were superovulated,mouse embryos of 2-cell and blastocysts were cultured with different concentration of hydrosalpinx fluid respectively.

• 方法收集人输卵管积水,小鼠促超排卵,收集2细胞胚胎和囊胚,随机分配到含有不同浓度输卵管积水的培养液中,观察胚胎的发育,计算成囊率,囊胚孵出率。