Methods: BALB/C female mice model of POF was established by multiple sites subcutaneous injection of ovarian antigen elicited from ovarian tissue of mature SD female rats.

• 方法：以成熟的SD雌性大鼠卵巢组织制备卵巢抗原，皮下多点注射免疫BALB/C雌性小鼠，建立卵巢早衰模型。