Methods: Aplly the EBN theory to 1,600 cases of our department, put up the chinical practice from the three aspects of vein selection, puncture method and the connecting tube method after puncture.

• 方法对在我科静脉采血的1600例患者应用循证护理原理，从血管选择、穿刺方法、穿刺后接管方法三方面进行临床实践。