Methods: Anti-CVB 3 effects were investigated by observing cytopathic effect (CPE), by using MTT colorimetric assay for viable cell rate, and by detecting CVB 3 -RNA using semi-quantitative RT-PCR.

• 方法 :通过观察细胞病变效应 (CPE) ,噻唑蓝 (MTT)比色法检测细胞活性 ,用半定量RT PCR检测不同药物浓度下病毒核酸含量 ,以确定有效单体抗CVB3 的作用。