Methods: 1. The ECV-304 monolayers were subjected to hypoxia for 6h in ECs at 4 ? followed by Oh 1h 2h 4h 6h 12h reoxygenation, respectively.

• 一、将培养至融合待用的ECV-304细胞给予低温(4℃)ECs缺氧保存6h，在给予复氧0h，1h，2h，4h，6h，12h。