Methods: (1) Using polymerase chain reaction(PCR) combined with single strand conformation polymorphism (SSCP) method and DNA sequencing to detect the point mutation of keratin 14 in a pedigree of DM-EBS.

• 采用多聚酶链反应(PCR)、单链构象多态性(SSCP)分析、DNA直接测序方法，对DM-EBS一家系角蛋白14(K14)进行基因分析。