Methods under the same condition with hematopoietic factors in the liquid culture, compared the sequential expansion group and once for all expansion group with or without stroma celllayer.

• 方法在相同细胞因子组合下，用体外液体培养比较脐血有核细胞程序扩增组与一次扩增组及有无基质层条件下的优劣。