Methods Whole cell patch clamp techniques were employed to investigate the effect of aminophylline on L-type calcium cur-rent of diaphragmatic muscles in rats.

• 方法:采用全细胞膜片钳（whole cell patchclamp）技术；当维持电位为-80mv；刺激频率为0.；5Hz；钳制时间为300ms；步进电压为10mv；去极到60mv；观察氨茶碱对膈肌细胞L型钙通道（L-ICa）的影响。