Methods Variation of P gene of HBV polymerase was determined by short segment PCR ELISA according to the recombined plasmid which was acquired by the site directed mutagensis.

• 方法 通过定点诱变获得的重组质粒作为标准对照 ,采用短片段PCR ELISA方法对拉米呋啶治疗的 6 0例乙肝患者血清标本进行HBV聚合酶P基因的变异检测 ,同时观察其HBVDNA定量、肝功能指标的变化。