Methods Three HLA class I-restricted HIV-1 epitope-specific tetramers were used to evaluate circulating specific CD+_8T cells in 14 HIV-1 infected subjects with positive HLA-A2 who were screened from 46 HIV-1 carriers using flow cytometry analyses.

• 方法应用三种tetramer检测14例HLA-A2阳性的HIV-1感染者外周血中特异性CTLs的频率,同时采用SL9(SLYNTVATL)体外冲击HIV-1感染者的外周血单核细胞(PBMCs)。