Methods The two microsatellite markers were amplified by polymerase chain reaction(PCR), followed by 10% non denatured polyacrylamide gel electrophresis (PAGE) and DNA sequencing to analyze the PCR products.

• 方法用聚合酶链反应（PCR）扩增与PKD1连锁的两个微卫星，10%25非变性聚丙烯酰胺凝胶电泳结合DNA序列测定检测PCR产物。