Methods The total proteins of hypothalamuses in the rats were separated by immobilized pH gradient-based two-dimensional gel electrophoresis. With coomassie blue staining,the 2-DE image was analyzed by PDQuest 7.0 software.

• 方法用固相pH梯度双向凝胶电泳分离肝阳上亢证大鼠与正常大鼠下丘脑的总蛋白质；经考马斯亮兰染色；PDQuest 7.；0软件分析。