Methods The tissue from subependymal region of adult Macaca fascicularis was digested in papain and DNase,screened through 300-mesh filter,then seeded into DMEM-F12 medium containing EGF and bFGF.

• 方法取成年食蟹猴脑室下区组织,经木瓜蛋白酶和脱氧核糖核酸酶消化后,接种于DMEM-F12无血清培养基中。