Methods The technology of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect MTHFR gene C677T polymorphism in 74 HD patients and 30 healthy controls.

• 方法 利用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)分析法检测了首都医科大学附属北京同仁医院肾内科74例HD患者和30例健康者MTHFR C677T基因多态性。