Methods The polymorphism of the ApoE gene was detected using rapid-cycle polymerase chain reaction(PCR) and fluorescence resonance energy transfer(FRET) technique for 25 patients with AD and 59 normal controls.

• 方法 应用快速PCR方法与荧光能量共振转移(FRET)相结合对25例AD患者及59名健康老人的ApoE基因进行检测，对AD患者的ApoE基因多态性进行相关分析。