Methods The pig skin was treated with negative pressure by a self-made device to remove the blistered epidermis,then dermal cells were removed by ion detergent with osmotic pressure gradient effects.

• 方法:利用自制的装置,先将猪皮经负压处理,去除起泡后的表皮,然后利用离子型去污剂的渗透压梯度作用去除真皮内的细胞成分。