Methods The islets were isolated and purificated by collagenase perfusion method,and counted by dithizone staining.The standard of isolated live cells was justified by trypan blue staining.

• 方法通过用胶原酶灌注分离纯化胰岛,用双硫棕染色,台盼蓝染色和胰岛素释放试验来鉴定胰岛的数量、形态和功能。