Methods The islets of NMRI mice were isolated by digestion combined with collagenase and DNase, incubated in RPMI 1640 tissue culture medium overnight. The experiments were completed with Millipore Multiscreen Assay System.

• 方法 胶原酶和DNA酶联合消化法分离NMRI小鼠胰岛 ,RPMI1 640组织培养液过夜培养 ,采用MilliporeMultiscreen系统观察CDS对胰岛功能的影响。